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Table 1 Biochemical parameters of Azospirillum brasilense Sp245 under various physiological conditions ranging from non-stressed to stressed conditions reflecting the physiological competence of the strain

From: Genome-wide identification of Azospirillum brasilense Sp245 small RNAs responsive to nitrogen starvation and likely involvement in plant-microbe interactions

Set

Physiological conditions

μg IAA/OD (IAA production)

μg PHB/mg cell biomass (PHB accumulation)

OD560 per cellular OD560 -biofilm units (Quantitative Biofilm formation)

nmol/h (Ethylene Production, Nitrogenase activity)

N1

C + N

16.27 ± 0.046

11.3 ± 0.2

11.94 ± 1.26

0.05

N2

C/2 + N/2

10.02 ± 0.053 a

54.2 ± 0.4 a

13.02 ± 1.86

0.72 a

N3

C + N/2

14.34 ± 0.028 a

42.4 ± 0.4 a

13.68 ± 0.98

0.75 a

N4

C-N

   

0.39 a

  1. The values represent the average of three independent sets ± standard error from the mean
  2. Statistical analysis was performed as described in Materials and methods
  3. a, Values significantly different from strain Sp245 grown in set N1 at full strength C + N at P ≤ 0.05
  4. IAA was quantified as mentioned previously. 1 mM Trp was added to each culture at the time of subculture and the respective substrates were added after 6 h of growth