Table 1 CRISPR gRNA target sequences and AmpliSeq primer sequences

Delta6abc/5

Δ6fads2-a

GGCACCGACAGAGCC

CAGCCAGG^a

Forward (5′- > 3′): TTTGTAGGACGCATTTGTCGC

Reverse (5′- > 3′): AGATGACACACTACTTTTCTAGGAG

Delta6abc/5

Δ6fads2-b

GGCACCGACAGAGCCCAGCCAGG^a

Forward (5′- > 3′): CCCGGGTCCCTACCTAAACCTA

Reverse (5′- > 3′): CTCCTCCCCTTCATCAGGTGAC

Delta6abc/5

Δ6fads2-c

GGCACCGACAGAGCCCAGCCAGG^a

Forward (5′- > 3′): GAGACGCTCTAGGCTTCACA

Reverse (5′- > 3′): TCCCAGCGGTTTGGATCATTC

Delta6bc

Δ6fads2-b

^aCCAAGGGTGGCGTGG

TTGGGCCC

Forward (5′- > 3′): TGATCCAAACCGCTGGGAAAT

Reverse (5′- > 3′): ACGGTGTGAGTGGAGCAGAG

Delta6bc

Δ6fads2-c

^aCCAAGGGTGGCGTGG

TTGGGCCC

Forward (5′- > 3′): AGAGTCCATTCCCAGGACGAA

Reverse (5′- > 3′): ACAGACTGGACAGAGCGTAG

Slc45a2

slc45a2

GGGGAACAGGCCGAT

AAGACTGG^a

Forward (5′- > 3′): TGTATGAGCTACAGACAGGTGG Reverse (5′- > 3′): AGGGGCTCTACTTCGTAGGAT