Fig. 2

Examples of data integration for homogeneous and heterogeneous NGS data. a Identification of open chromatin regions near Eef2 gene using ATAC-Seq. Tracks for replicates of ATAC-Seq data from mpkCCD cells were integrated into a single track using NGS-Integrator (window size: 10 kb, background: 2× median across the window). Eef2 gene, coding for Elongation Factor 2 (EF-2), is a housekeeping gene and mainly involved in the GTP-dependent ribosomal translocation step during translation elongation. Original data tracks in black; integrated data track in red. b Identification of active enhancer regions near Eef2 gene using multiple NGS techniques. Three different NGS datasets were generated using data from ATAC-Seq, ChIP-Seq for histone H3K27Ac and ChIP-Seq for RNA Polymerase II in mouse kidney cortical collecting duct cell mpkCCD. The datasets were integrated to identify potential regulatory elements (window size: 10 kb, background: 2× median across the window). Original data tracks in black; integrated track in blue. All data tracks were displayed on the UCSC Genome Browser with mouse genome mm10