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Fig. 1 | BMC Genomics

Fig. 1

From: Detection of CRISPR-mediated genome modifications through altered methylation patterns of CpG islands

Fig. 1

Mouse strain edit sites. The methylation patterns of three CRISPR-edited animal strains were evaluated in this study, describe in detail in Table 1. Strain HDR1 (a) contains an 11-kb insert and was recombinantly inserted into the Rxfp3 locus of the mouse genome using a 1-kb upstream homology arm (UHA) and a 4-kb downstream homology arm (DHA). The protospacer adjacent motif (PAM) site was located at Chr15:11,037,280. Strain HDR2 (b) contains a 2005-bp insert and was recombinantly inserted into the Rosa26 locus of the mouse genome using a 1-kb UHA and a 1-kb DHA. The PAM site was located at Chr6:113,076,025. Strain NHEJ1 (c) contains a 48-bp duplex DNA oligonucleotide fragment incorporated into the Rosa26 locus by non-homologous end-joining. The PAM site was located at Chr6:113,076,025. For each figure, the yellow bar indicates the CGI location

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