Table 1 Mouse strains

Control 1

005304

Wild-type parental strain

C57Bl/6NJ

Control 2

000664

Wild-type parental strain

C57Bl/6 J

HDR1

028551

CRISPR-mediated HDR insertion using the pRosa-Cas9 targeting vector with an 11-kb insert flanked by Rosa26 locus homology regions, extending 1 kb upstream and 4 kb downstream from the XbaI site within exon 1. The insert contained a codon-optimized Cas9 gene linked to an IRES-GFP.

C57B1/6 J-Gt(ROSA)26Sor^{em1(CAG-cas9*,-EGFP)Rsky}/J

HDR2

028543

CRISPR-mediated HDR insertion of a 2005-bp donor DNA containing eGFP with a poly A signal in an inverted orientation at the start codon of the Rxfp3 locus. The original insertion vector had homology arms of 1 kb, flanking the insertion sequence.

C57BL/6NJ-Rxfp3^em2J/J

NHEJ1

028573

CRISPR-mediated NHEJ insertion of a 48 base pair duplex DNA oligo with no homology arms into the Rosa26 locus. During endogenous DNA repair, a serendipitous indel of −3 bp and + 6 bp occurred.

C57BL/6 J-Gt(ROSA)26Sor^em2Mvw/Mvw