|
Control 1
|
005304
|
Wild-type parental strain
|
C57Bl/6NJ
|
|
Control 2
|
000664
|
Wild-type parental strain
|
C57Bl/6 J
|
|
HDR1
|
028551
|
CRISPR-mediated HDR insertion using the pRosa-Cas9 targeting vector with an 11-kb insert flanked by Rosa26 locus homology regions, extending 1 kb upstream and 4 kb downstream from the XbaI site within exon 1. The insert contained a codon-optimized Cas9 gene linked to an IRES-GFP.
|
C57B1/6 J-Gt(ROSA)26Sorem1(CAG-cas9*,-EGFP)Rsky/J
|
|
HDR2
|
028543
|
CRISPR-mediated HDR insertion of a 2005-bp donor DNA containing eGFP with a poly A signal in an inverted orientation at the start codon of the Rxfp3 locus. The original insertion vector had homology arms of 1 kb, flanking the insertion sequence.
|
C57BL/6NJ-Rxfp3em2J/J
|
|
NHEJ1
|
028573
|
CRISPR-mediated NHEJ insertion of a 48 base pair duplex DNA oligo with no homology arms into the Rosa26 locus. During endogenous DNA repair, a serendipitous indel of −3 bp and + 6 bp occurred.
|
C57BL/6 J-Gt(ROSA)26Sorem2Mvw/Mvw
|
