Fig. 4

Impact of transcriptional profile changes after parturition and the onset of lactation on upstream regulators and biological functions as predicted by IPA analysis. Network analyses combined differentially expressed genes with upstream regulators and physiological functions to identify factors related to the activation or inactivation of lipid mobilization biological processes (p ≤ 0.05). After parturition, hubs in the networks colored orange are predicted to have activation, and those in blue are predicted to be inhibited. Color lines connecting hubs indicate IPA prediction of hubs whose activity lead to upregulation (orange line) or downregulation (blue line). Networks analyzed: (a) Release of lipids; (b) Inhibition of long-chain fatty acids synthesis; (c) Inhibition of lipid synthesis of lipids