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Fig. 4 | BMC Genomics

Fig. 4

From: Exploring protocol bias in airway microbiome studies: one versus two PCR steps and 16S rRNA gene region V3 V4 versus V4

Fig. 4

The 20 most abundant amplicon sequence variants (ASVs) observed in negative control samples (NCS) after sequencing following setup 1. Multiple ASVs mapped to genera Gluconacetobacter, belonging to familiy Enterobacteriaceae (cummulative 22%). Bioinformatic processing steps were performed up until the removal of contaminants identified using Decontam. Taxonomic rank is described using prefixes (c__: class, o__: order, f__: family, g__: genus). Data is presented as the average relative abundance. Data unrarefied

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