Fig. 5

Taxonomic distribution obtained for procedural samples before the removal of Decontam contaminants. ASVs attributed to the family Enterobacteriaceae, had dominated the NCS across all setups. In the procedural samples, Enterobacteriaceae was observed with the following relative abundances in setups 2 and 3: setup 2 (OW: 0%; PBAL: 0.83%; PSB: 5.23%); setup 3 (OW: 0.01%; PBAL: 1.87%; PSB: 7.51%). ASVs attributed to the genus Gluconacetobacter within the family Enterobacteriaceae was observed in procedural samples with the following relative abundances in setup 1 (OW: 0%; PBAL: 1.42%; PSB: 6.32%). Samples with fewer than 1000 sequences had been omitted from the analyses leaving the following number of samples in each setup: Setup 1 (OW: n = 22; PBAL: n = 23; PSB: n = 23); Setup 2 (OW: n = 23; PBAL: n = 23; PSB: n = 23); Setup 3 (OW: n = 23; PBAL: n = 21; PSB: n = 22). Setup 1 (2-step PCR; V3 V4 region); Setup 2 (2-step PCR; V4 region); Setup 3 (1-step PCR; V4 region). Taxonomic rank is described using prefixes (p__: phyla; c__: class; o__: order; f__: family; g__: genus)