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Fig. 6 | BMC Genomics

Fig. 6

From: Exploring protocol bias in airway microbiome studies: one versus two PCR steps and 16S rRNA gene region V3 V4 versus V4

Fig. 6

Taxonomic distribution obtained for procedural samples after the removal of Decontam contaminants. Samples with fewer than 1000 sequences were omitted. Number of samples in each setup: V3V4 protocol A (OW: n = 22; PBAL: n = 22; PSB: n = 21), V4 protocol A (OW: n = 23; PBAL: n = 22; PSB: n = 20); V4 protocol B (OW: n = 23; PBAL: n = 21; PSB: n = 21). Setup 1 (2-step PCR; V3 V4 region); Setup 2 (2-step PCR; V4 region); Setup 3 (1-step PCR; V4 region). Taxonomic rank is described using prefixes (p__: phyla; c__: class; o__: order; f__: family; g__: genus)

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BMC Genomics

ISSN: 1471-2164

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