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Fig. 7 | BMC Genomics

Fig. 7

From: Exploring protocol bias in airway microbiome studies: one versus two PCR steps and 16S rRNA gene region V3 V4 versus V4

Fig. 7

Principal coordinates analysis on unweighted UniFrac distances for procedural samples sequenced following setup 2 (sphere) and 3 (diamond) before the removal of Decontam contaminants. Rarefaction depth: 1066 sequences. Setup 2 samples include OW: n = 23; PBAL: n = 23; PSB: n = 23; NCS: n = 21 and setup 3 samples include OW: n = 23; PBAL: n = 21; PSB: n = 22; NCS: n = 18. Oral Wash (OW): blue; Protected bronchoalveolar lavage (PBAL): green; Protected specimen brushes (PSB): purple; Negative control samples (NCS): red. Setup 2 (2-step PCR; V4 region), Setup 3 (1-step PCR; V4 region)

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BMC Genomics

ISSN: 1471-2164

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