Fig. 4From: Intricate genetic variation networks control the adventitious root growth angle in appleAllelic variation of MdNPR5, effects on promoter activity, and function in adventitious RGA in ‘BC’, ‘M9’ and their F1 hybrids. a MdNPR5 expression by RT-qPCR (bar chart) and mean FPKM values (line chart) during adventitious root formation in hybrids with small (S) (13–0670, 13–0925, and 13–1611) and large (L) (12–1529, 12–1585, and 12–1830) RGAs. b Schematic representation of single nucleotide variation within the promoter of MdNPR5. c Box plots showing differences in RGA between hybrids with the C:T and C:C genotypes of marker S1272. Numbers of hybrids are presented in parentheses. d Schematic of promoter truncations of MdLNPR5-pro: LUC vector constructs with or without the LBD cis-element. e Transient expression analysis of various MdNPR5-pro: LUC constructs. “pro” represents the promoter. f Subcellular localization of transiently expressed MdNPR5:GFP in N. benthamiana leaves. Scale bars = 50 mm. g RGA phenotypes of the 35S:MdNPR5 transgenic N. benthamiana lines and untransformed wild type. The RGA values are presented in parentheses. h and (i) Relative expression of MdLBD41 and MdbHLH48 when MdNPR5 was transiently expressed by the vectors 35S:MdNPR5 (h) and pTRV:MdNPR5 (i). Asterisks represent P < 0.05 by Dunnett’s multiple comparisonBack to article page