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Fig. 4 | BMC Genomics

Fig. 4

From: Intricate genetic variation networks control the adventitious root growth angle in apple

Fig. 4

Allelic variation of MdNPR5, effects on promoter activity, and function in adventitious RGA in ‘BC’, ‘M9’ and their F1 hybrids. a MdNPR5 expression by RT-qPCR (bar chart) and mean FPKM values (line chart) during adventitious root formation in hybrids with small (S) (13–0670, 13–0925, and 13–1611) and large (L) (12–1529, 12–1585, and 12–1830) RGAs. b Schematic representation of single nucleotide variation within the promoter of MdNPR5. c Box plots showing differences in RGA between hybrids with the C:T and C:C genotypes of marker S1272. Numbers of hybrids are presented in parentheses. d Schematic of promoter truncations of MdLNPR5-pro: LUC vector constructs with or without the LBD cis-element. e Transient expression analysis of various MdNPR5-pro: LUC constructs. “pro” represents the promoter. f Subcellular localization of transiently expressed MdNPR5:GFP in N. benthamiana leaves. Scale bars = 50 mm. g RGA phenotypes of the 35S:MdNPR5 transgenic N. benthamiana lines and untransformed wild type. The RGA values are presented in parentheses. h and (i) Relative expression of MdLBD41 and MdbHLH48 when MdNPR5 was transiently expressed by the vectors 35S:MdNPR5 (h) and pTRV:MdNPR5 (i). Asterisks represent P < 0.05 by Dunnett’s multiple comparison

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