Fig. 8From: New insights into the evolution and functional divergence of the CIPK gene family in SaccharumThe effect of transient overexpression of ScCIPKs in Nicotiana benthamiana leaves after inoculation with Ralstonia solanacearum. A, RT-PCR analysis (a) (The gels were selected from the same gel with the same exposure, and the unmodified figure was shown in the Fig. S4-S7) and the relative transcript levels of the immunity-associated marker genes (b) in the ScCIPKs transiently expressed N. benthamiana challenged by R. solanacearum for 1 day. B, RT-PCR analysis (a) (The gels were selected from the same gel with the same exposure, and the unmodified figure was shown in the Fig. S4-S7) and the relative transcript levels of the immunity-associated marker genes (b) in the ScCIPKs transiently expressed N. benthamiana challenged by R. solanacearum for 7 days. NtEF1-α was used for normalization of the transcript levels. Mock, the Agrobacterium strain carrying 35S::00. All of the data points were expressed as the mean ± SE (n = 3). Different lowercase letters indicate a significant difference between 35S::00 and 35S::ScCIPKs in each gene, as determined by the Duncan’s new multiple range test (p-value < 0.05)Back to article page