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Fig. 8 | BMC Genomics

Fig. 8

From: New insights into the evolution and functional divergence of the CIPK gene family in Saccharum

Fig. 8

The effect of transient overexpression of ScCIPKs in Nicotiana benthamiana leaves after inoculation with Ralstonia solanacearum. A, RT-PCR analysis (a) (The gels were selected from the same gel with the same exposure, and the unmodified figure was shown in the Fig. S4-S7) and the relative transcript levels of the immunity-associated marker genes (b) in the ScCIPKs transiently expressed N. benthamiana challenged by R. solanacearum for 1 day. B, RT-PCR analysis (a) (The gels were selected from the same gel with the same exposure, and the unmodified figure was shown in the Fig. S4-S7) and the relative transcript levels of the immunity-associated marker genes (b) in the ScCIPKs transiently expressed N. benthamiana challenged by R. solanacearum for 7 days. NtEF1-α was used for normalization of the transcript levels. Mock, the Agrobacterium strain carrying 35S::00. All of the data points were expressed as the mean ± SE (n = 3). Different lowercase letters indicate a significant difference between 35S::00 and 35S::ScCIPKs in each gene, as determined by the Duncan’s new multiple range test (p-value < 0.05)

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