Fig. 1

Phylogenetic relationships of Brassica napus NPF proteins (BnaNPFs) and Arabidopsis NPF proteins (AtNPFs). The neighbor-joining tree (NJ) was built using the full-length NPF proteins in Arabidopsis (53) and B. napus (155); NPFs were classified into 11 subfamilies (S1, S2–1, S2–2, S3, S4, S5–1, S5–2, S6–1, S6–2, S7, and S8). The bootstrap value of each subfamily is marked in the tree with a black dot. The substrates of AtNPFs that had been functionally demonstrated are indicated in the subfamilies with colored dots, and the main substrates of each subfamily are summarized along the outer circle. IAA: indoleacetic acid, MeJA: methyl jasmonate, ABA: abscisic acid, GA: gibberellic acid, MTB: methylthiobutyl glucosinolate. The neighbor-joining tree was constructed by MEGA7.0 software and visualized and edited in Evolview V3