Fig. 1

Summary of Iso-seq data. a CCS read length distribution, the X-axis represents the read length, the Y-axis on the left represents the column graph coordinate, indicating the number of reads with a certain length (X-axis), and the Y-axis on the right is the curve coordinate, indicating the number of reads whose length is bigger than a certain X-axis value. b The percentage of high quality isoforms mapped to the reference genome. c Classification and number of isoforms mapped to the reference genome. Known isoform was mapped to annotated genes in reference genome, new isoform was mapped to different exons of annotated gene in reference genome, and novel isoform was mapped to unannotated region in reference genome. d and e The number of genes and transcripts annotated in reference genome and Iso-seq data. f BUSCO assessment results of reference genome and optimized genome of spinach. Ref, reference genome; Ref + Iso, genome optimized via PacBio Iso-seq sequencing