Fig. 3

Comparison of sequence capture, ARTIC v3 amplicon, and tailed amplicon workflows on clinical specimens spanning a range of viral loads. a Samples with N1 and N2 Ct values ranging from approximately 20–35 chosen for testing of SARS-CoV-2 sequencing workflows. Samples are colored as in panels c-f. b Evenness of representation of amplicons for different workflows as a function of sample N1 Ct value. Percentage of genome coverage at 100x at different subsampled read depths for each sample when sequenced using the following approaches: c Illumina Nextera DNA Enrichment; d ARTIC v3 with TruSeq library preparation. e Tailed amplicon v1 (2 pool amplification); f Tailed amplicon v2 (4 pool amplification)