Fig. 12

Cl⁻ channel subtypes and families identified in the L. stagnalis CNS transcriptome assembly. The tree was generated as described in Fig. 8, where the analysis involved 18 amino acid sequences and yielded a total of 1233 positions in the final dataset, resulting in a tree with highest log likelihood of − 22,161.37. The accession numbers and BLAST information of transcripts represented in this tree are provided in Table S26. A variety of putative L. stagnalis Cl⁻ channel subtypes and families are identified, of which anoctamin, bestrophin, and intracellular chloride channel protein have not yet been cloned from L. stagnalis