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Fig. 8 | BMC Genomics

Fig. 8

From: Transcriptome profiling revealed early vascular smooth muscle cell gene activation following focal ischemic stroke in female rats – comparisons with males

Fig. 8

Validation of gene targets in male and female middle cerebral arteries (MCAs) after tMCAO (continued). Differential gene expression of the selected upregulated gene targets was validated by qPCR. When comparing delta cycle threshold (dCT) values between the (non-operated) control MCAs, and the non-occluded and occluded MCAs after tMCAO [transient middle cerebral artery occlusion], significant elevations were seen in both sexes for sphingosine 1 phosphate receptor 3 (S1pr3), suppressor of cytokine signaling 3 (Socs3), Jun B proto-oncogene (JunB), and Fos-like antigen 1 (Fosl1). Sex did not significantly affect the outcome for any of the genes being validated (p = 0.11–0.87) therefore, only the fixed effect 'experimental group' was fitted into the model (p < 0.01). First, we compared the occluded and non-occluded MCAs to the reference ‘control MCAs’, and second, we compared the occluded MCAs to the reference ‘non-occluded MCAs’. Information within parentheses shows number of samples for the sex (F/M) and group in question. Statistical tests were performed (see methods): * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 and NS indicates non-significance (p > 0.05). Abbreviations: F, female; M, Male

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