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Fig. 5 | BMC Genomics

Fig. 5

From: RNA sequencing identifies transcriptional changes in the rabbit larynx in response to low humidity challenge

Fig. 5

RT-qPCR validation. Relative quantification for each gene was determined by the ΔΔCt method. All reactions were run in triplicate. The level of expression of each tested gene was standardized to the housekeeping gene HPRT1, and ΔΔCt was calculated using the average of the ΔCts from the control group for the respective gene. ECCP, MCP1 and MMP12 were significantly different (p < 0.05) and SPBN and ZACN marginally non-significant (p = 0.06). Differences between groups as determined by the Welch t-test. Results represent 5–7 samples/group for each gene after the removal of outlier values as determined by the iterative application of a two-tailed Grubb’s test. Error bars represent the SEM for relative quantification within the respective humidity group

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