Phylogenetic analysis and determination of ANI, dDDH, and CGASI values of 7 representative Ehrlichia species. a A maximum-likelihood phylogenetic tree with 1,000 bootstraps was generating using the core genome alignment used to calculate CGASI values. Bootstrap values are indicated next to their respective nodes. b The values of ANI, dDDH, and CGASI are calculated between 7 Ehrlichia genomes and plotted as a heatmap. The respective values for each pairwise comparison are shown in each cell. Colored circles next to each strain name indicate whether each genome belongs to the same species, with circles of the same color indicating genomes are of the same species according to either ANI, dDDH, or CGASI below the species cutoffs of 95%, 70%, and 96.8%, respectively. Abbreviations and GenBank Accession numbers: EHF, Ehrlichia sp. HF (NZ_CP007474.1); EchA, E. chaffeensis Arkansas (NC_007799.1); EmuA, E. muris subsp. muris AS145 (NC_023063.1); EmuW, E. muris subsp. eauclairensis Wisconsin (NZ_LANU01000001, NZ_LANU01000002, and NZ_LANU01000003); EcaJ, E. canis Jake (NC_007354.1); EruW, E. ruminantium Welgevonden (NC_005295.2); EruG, E. ruminantium Gardel (NC_006831.1).