Fig. 3

Differential expressed genes (DEGs) in whole brain transcriptomes in proactive S. salar, D. labrax and D. rerio. a-i ~ iii) Volcano plots show DEGs, up-regulated DEGs in the proactive group are shown in red and the control group in blue (log2 (Fold Change)” > 0, and “false discovery rate (FDR)” < 0.05). b-i ~ iii) Hierarchical clustering of DEGs and samples. Heatmaps show the relative expression levels of each DEGs (rows) in each sample (column). Expression values (FPKM for both S. salar and D. labrax and probe intensities for D. rerio are log2-transformed and then median-centred by DEGs. c-i ~ iii) DEG clusters extracted from the hierarchical clustering. X axis: samples; y axis: median-centred log2. Individual DEGs are shown as grey lines; the average expression values per cluster are shown as blue lines. No. of up-regulated DEGs in the proactive group from each cluster is shown in red, control group DEGs are blue. Abbreviations: “S” or “L” are “S. salar” or “D. labrax” respectively; 2nd letter “P” = “Pool”; Numeric lettering indicates replicates within group; 3rd lettering “P” or “C” is “Proactive” or “Control”)