Fig. 2

Fate of ingested synthetic long dsRNA. a Alignment of reads to the Actin mRNA (CAEY01002033.1) sequenced from column purified RNAs following feeding with dsRNA (top panel), no feeding after column purification (middle panel), and total RNA from unfed mites (bottom panel). Red dashes are positive strand mapping reads, while blue represent negative strand mapping reads. Grey, overlaid density plots represent total read accumulations. b-c. Number of Dicer small RNA sequencing read pairs of different sizes derived from dsRNA targeted to GFP (b) or Actin (c). Pairs are defined by overlapping by 2 nt less than the length of the query strand of the pair. This simulates the 2 nt overhangs found after Dicer processing of dsRNA. Duplexes are biases toward short (18-22 nt) pairs that show asymmetry by one or two bases d Percent of plus or minus strand mapping in Total RNA (from bottom panel of 2a), dsRNA targeted region following feeding and column purification (top panel 2a), or flanking region when dsRNA is fed and column purification is used (top panel 2a). e-f Seqlogo plot showing sequence bias for reads mapping to dsRNA flanking region (top panel 2a) (e) or minus strand (f) of Actin