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Fig. 4 | BMC Genomics

Fig. 4

From: Environmental RNAi pathways in the two-spotted spider mite

Fig. 4

Chloroplast mapping RNAs. a Coverage of chloroplast relative to nuclear genome for RNAs recovered from total and HiTrap purified RNAs from mites raised on bean plants, bean plants after dsRNA feeding, and A.thaliana. b Plastid genome coverage occuring on both strands for a specific region or only on a single strand. c Distribution of reads mapping to P.vulgaris chloroplast genome. Top trace shows chloroplast genes. Next panel, alignment of individual reads from HiTrap purified samples with red spots corresponding to plus strand and blue negative strand mapping. Bottom two panels show read densities for HiTrap and total RNA samples. Orange represents 19-23 nt reads that map in multiple positions, and blue unique mapping positions for the 19-23 nt size range. Yellow shows densities of all size ranges (15-30 nt) d Density plots showing read accumulation in the region in (c) highlighted by the gray dashed box. Library used to generate plot above, mapping separated by strand. Red represents all read ranges (15-30 nt), and blue siRNA size only (19-23 nt). Y-axis is maximum density

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