Fig. 6

Quantitative real-time PCR analysis of Md14–3-3 gene expression in apple buds treated with gibberellin (GA₃). GA₃ at 500 mg L^− 1 was sprayed on ‘Nagafu No. 2’ trees in a clear morning at 25 and 30 DAFB. At the same time, water was sprayed as a control. After treated twice, samples of the spur terminal buds were collected at 30, 50, and 70 days after full bloom (DAFB). Each value represents the standard error of three replicates. The expression profiles of genes were compared in the context of the gene relationships (the non-ε group on the left and the ε group on the right). The significance of the difference between the control and treatment groups was estimated by Student t-test at each date (*, p-value < 0.05)