Fig. 3

Age- and state-dependent antennal transcript abundance. a Principal component analysis of the antennal transcriptomes of non-blood fed (nbf; circles) and blood fed (bf; squares) female Aedes aegypti, 5 to 10 days post-emergence (dpe). Females were blood fed 5 dpe and the time is represented as hours post-blood meal (pbm). Ages are denoted by a gradient of green hues, with the lightest being 5 dpe and the darkest being 10 dpe. Inset: The area bordered by dotted grey lines is expanded for disambiguation. Three replicates of each antennal transcriptome for nbf and bf are depicted for each age. b Total number of genes with differentially abundant transcripts between the antennal transcriptomes of nbf and bf from 5 to 10 dpe adult female Ae. aegypti can be determined by the sum of those with gene ontology (GO) annotation (white) and those without (green). c Proportions of genes with differentially abundant transcripts in the antennae of age-matched host-seeking (nbf) and blood-fed (h pbm) adult female Ae. aegypti between 5 to 10 days post-emergence (dpe) were classified by a level 3 molecular function GO. The legend indicates the GO terms representing ≥2% of the total differentially abundant transcripts in at least one pairwise comparison