Table 1 Primers used in real-time PCR analysis
Genes | Primers | Size (BP) | Annealing T (°C) | Accession N0. |
|---|---|---|---|---|
F13A1 | F: GGATGCTTTTGGTCTGATAC | 100 | 55 °C | NM_204685.1 |
R: CTGCTTGTTGATCTCATTGG | ||||
CLU | F: CGTCAGTTCGGTTGGGTCTT | 100 | 55 °C | NM_204900.1 |
R: CCTCGAGGTTGGGAGTTTTG | ||||
TBXA2R | F: GACAGCGAAGTGGAGATGAT | 100 | 55 °C | XM_015299775.1 |
R: GGAGAGTGGTCTGGATGATG | ||||
IL1R1 | F: TTTCACGCACCATGAATCTG | 100 | 55 °C | NM_205485.1 |
R: GAGCCGAGTTCCACTTCAAT | ||||
RPL17 | F: GTGAACAAGGCTCCCAAAAT | 100 | 55 °C | NM_001282277.1 |
R: CGGTGAGGATCATCTCAATG | ||||
ITGB3 | F: TTTGTGGACAAGCCCATTTC | 100 | 55 °C | NM_204315.1 |
R: CAAACATGGGCAAGCACTTT | ||||
ITGAV | F: TCAGTGGTTTGGAGCATCTG | 100 | 55 °C | NM_205439.1 |
R: AGGCTCTCGCTCTTGTTTTG | ||||
ITGB2 | F: CAGTGCGTTCAGCAATAAGA | 100 | 55 °C | NM_205251.1 |
R: AGCACGACGAAACTTCACAT | ||||
RAC2 | F: GGAGAATACATCCCCACTGT | 100 | 55 °C | NM_001201452.1 |
R: CCTCTTGTCCAGCAGTATCC | ||||
ITGA2 | F: TGCTAATAATCCCCGTGTAG | 100 | 55 °C | XM_015277561.1 |
R: CAGATCTCCTCCCTGTTGAA | ||||
IQGAP2 | F: CTGGTGAGGCCAGTAAATTG | 100 | 55 °C | NM_001277778.1 |
R: CGCAAACTCTGAATCGATGA | ||||
GIT1 | F: CATCACGCTGCAGGAGTACCT | 100 | 55 °C | NM_204296.1 |
R: CTCATCGCTCAGGCTGTTGT | ||||
VAV1 | F: CTGGAGACGTTGTAGGGTATG | 100 | 55 °C | NT_469922.1 |
R: TGGTGAGACGGGTTGGTT | ||||
ITGA5 | F: CTCCAACTACCCCGAGTACT | 100 | 55 °C | KC439457.1 |
R: CACCGAATAGCCCATATAAC | ||||
RAP1B | F: TCGAGTCAAAGACACTGATG | 100 | 55 °C | NM_001007852.1 |
R: TAGGTTCTGACCTTGTTCCT | ||||
FYN | F: AGGAGTGGTACTTCGGCAAA | 100 | 55 °C | NM_205349.2 |
R: GTTTCACTTTCCCGGATCAG | ||||
PTPN11 | F: TGGAGGCAGAAAATCTACTG | 100 | 55 °C | NM_204968.1 |
R: CGCCTAACAGAGAGAGTGAA | ||||
PTCH1 | F: TTCCTTCTAGCCCATGCGTTT | 100 | 55 °C | NM_204960.2 |
R: CTACACTTGCTCCTGTTCGCTTT | ||||
NCOR2 | F: GGTCCCACACACTTGAC | 100 | 55 °C | XM_015275621.1 |
R: GGTGGATACTAGGATGGATT | ||||
MAML3 | F: GCAACCACACGCTGATCATG | 100 | 55 °C | XM_015276692.1 |
R: CCATCGCAAACTCCATTCTG |