Fig. 1
M6A level of circRNAs in HPH rats and the number of m6A peak in circRNAs Rats were maintained in a normobaric normoxic (N, FiO2 21%) or hypoxic (HPH, FiO2 10%) chamber for 3 weeks, then RVSP was detected (a, b). c The ratio of RV/ (LV + S). d H&E staining and immunohistochemical staining of α-SMA were performed in the lung sections. Representative images of pulmonary small arteries. Scale bar = 50 μm. Quantification of wall thickness (e) and vessel muscularization (f). g Heatmap depicting hierarchical clustering of altered m6A circRNAs in lungs of N and HPH rats. Red represents higher expression and yellow represents lower expression level. h Box-plot for m6A peaks enrichment in circRNAs in N and HPH. i Distribution of the number of circRNAs (y axis) was plotted based on the number of m6A peaks in circRNAs (x axis) in N and HPH. Values are presented as means ± SD (n = 6 in each group). Only vessels with diameter between 30 and 90 μm were analyzed. NM, nonmuscularized vessels; PM, partially muscularized vessels; FM, fully muscularized vessels. **0.001 ≤ P ≤ 0.009 (different from N); ***P < 0.001 (different from N)