Fig. 5
Construction of a circRNA–miRNA–mRNA co-expression network in HPH (a) Comparison of the relationship between m6A level and expression of circRNAs between N and HPH. The fold-change ≥2.0 was considered to be significant, which was the m6A abundance of HPH relative to N. Red dots represents circRNAs with upregulated m6A level and blue dots represents circRNAs with downregulated m6A level. IP/Input referred to the m6A abundance in circRNAs detected in MeRIP-Seq (IP) normalized to that detected in input. b and c GO enrichment analysis includes BP analysis, CC analysis, and MF analysis. P values are calculated by DAVID tool. d and e KEGG signaling pathway analysis for the downstream mRNAs which was predicted to be ceRNA of DE cirRNAs. Methy. down & exp. down represents downregulated cirRNAs with decreased m6A level. Methy. up & exp. up represents upregulated cirRNAs with increased m6A level. f and g CeRNA analysis for DE circRNAs. Network map of circRNA-miRNA-mRNA interactions. Green V type node: miRNA; yellow circular node: DE circRNAs; blue hexagon node: target genes of miRNAs; red hexagon node: PH-related genes