Table 1 Ranking and statistical analyses of candidate reference genes in the skin samples from Tropheus duboisi. The selected reference genes (rps17, eif5a) are highlighted by grey shading. According to BestKeeper SD ranking, rpl34 and rpl8 displayed high variation across all the skin tissues (SD > 1.5) and were discarded from further consideration
From: Comparative transcriptomics reveals candidate carotenoid color genes in an East African cichlid fish
BestKeeper | geNorm | NormFinder | |||||
|---|---|---|---|---|---|---|---|
Ranks | SD | Ranks | r | Ranks | M | Ranks | SV |
rpl3 | 0.958 | eif5a | 0.989 | eif5a | 0.404 | eif5a | 0.113 |
rps17 | 0.981 | rps17 | 0.986 | rps17 | 0.406 | rps17 | 0.152 |
pabpc1b | 0.997 | eef1a1l1 | 0.984 | rpl3 | 0.425 | pabpc1b | 0.154 |
rpl30 | 1.046 | hspa8 | 0.983 | pabpc1b | 0.442 | eef1a1l1 | 0.169 |
eif5a | 1.128 | rpl3 | 0.983 | eef1a1l1 | 0.442 | rpl3 | 0.183 |
eef1a1l1 | 1.171 | pabpc1b | 0.978 | rpl30 | 0.484 | rpl30 | 0.190 |
ppiab | 1.175 | rpl30 | 0.938 | hspa8 | 0.486 | hspa8 | 0.211 |
hspa8 | 1.227 | ppiab | 0.927 | ppiab | 0.659 | ppiab | 0.257 |
rpl34 | 1.669 | rpl34 | – | rpl34 | – | rpl34 | – |
rpl8 | 1.850 | rpl8 | – | rpl8 | – | rpl8 | – |