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Table 1 Ranking and statistical analyses of candidate reference genes in the skin samples from Tropheus duboisi. The selected reference genes (rps17, eif5a) are highlighted by grey shading. According to BestKeeper SD ranking, rpl34 and rpl8 displayed high variation across all the skin tissues (SD > 1.5) and were discarded from further consideration

From: Comparative transcriptomics reveals candidate carotenoid color genes in an East African cichlid fish

BestKeeper geNorm NormFinder
Ranks SD Ranks r Ranks M Ranks SV
rpl3 0.958 eif5a 0.989 eif5a 0.404 eif5a 0.113
rps17 0.981 rps17 0.986 rps17 0.406 rps17 0.152
pabpc1b 0.997 eef1a1l1 0.984 rpl3 0.425 pabpc1b 0.154
rpl30 1.046 hspa8 0.983 pabpc1b 0.442 eef1a1l1 0.169
eif5a 1.128 rpl3 0.983 eef1a1l1 0.442 rpl3 0.183
eef1a1l1 1.171 pabpc1b 0.978 rpl30 0.484 rpl30 0.190
ppiab 1.175 rpl30 0.938 hspa8 0.486 hspa8 0.211
hspa8 1.227 ppiab 0.927 ppiab 0.659 ppiab 0.257
rpl34 1.669 rpl34 rpl34 rpl34
rpl8 1.850 rpl8 rpl8 rpl8
  1. Abbreviations: SD Standard deviation, SV Stability value, M Average expression stability value