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Table 1 Summary of the sequence assembly after transcriptome sequencing

From: Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

Sample

Raw_reads

Clean_reads

Clean_bases (Gb)

Error_rate (%)

Q20 (%)

Q30 (%)

GC (%)

SG

61,624,022

60,415,044

8.98

0.02

96.34

91.33

45.81

SC

57,374,966

55,414,904

8.15

0.02

95.22

90.11

44.08

ST

63,794,266

61,512,408

9.1

0.02

95.65

90.45

47.47

SZ

70,247,636

67,641,982

10.05

0.02

95.75

90.4

47.17

Total

253,040,890

244,984,338

36.28

–

–

–

–

  1. SG spermatogonium; SC spermatocyte; ST spermatid; SZ spermatozoa; Gb Giga base; Q20 percentage of bases with a Phred value of at least 20; Q30, percentage of bases with a Phred value of at least 30
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BMC Genomics

ISSN: 1471-2164

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