Table 3 The primers used in verifying the quality of samples
Primers | Purpose | Sequence (from 5’to 3′) |
|---|---|---|
ef1a-F | verifying | GGTCGTCACCTTCGCTCCAT |
ef1a-R | verifying | TCCCTTGGGTGGGTCATTCT |
dmrt1-F | verifying | GCTGGAGTAGACTGCTTGTTT |
dmrt1-R | verifying | CGACTGTGCGTCAGTATGAGC |
slbp2-F | verifying | CGAAGATGACCTCCGACCTG |
slbp2-R | verifying | CGGAGCCAGTCAGTCATGTT |
vasa-F | verifying | ACCAGATCTTCCTGGAGGC |
vasa-R | verifying | CAAATGACTGCTCCACGTCA |
zbtb16-F | verifying | GCAGGGGACCATCCATTTGA |
zbtb16-R | verifying | CACACGGTAGTGGGTTTCCA |
zbtb40-F1 | verifying | TTTGCTCACCCATCAGGCAT |
zbtb40-R1 | verifying | CCAAAGAGGCGAAGCTGAGA |
zbtb40-F2 | ISH | TCTCAGCTTCGCCTCTTTGG |
zbtb40-R2 | ISH | AGAGATCACTGACTCCGCCT |