Fig. 2

3’Pool-seq provides robust and reproducible gene expression quantification. a Read distribution from full-length mRNA-seq (Truseq) and 3’Pool-seq in the ApoE gene region. Reads generated using 3’Pool-seq are mapped preferentially towards the 3′-end of the gene. b Correlation of the abundance levels of ERCC spike-ins between 3’Pool-seq quantifications and actual pre-mixed concentrations. c Correlation of the abundance levels of ERCC spike-ins between 3’Pool-seq replicates. d Correlation of gene expression values (log₂TPM) between 3’Pool-seq replicates. e Number of genes detected with different minimal abundance thresholds at increasing read depths (i.e. total number of reads uniquely aligned to gene features). f Distribution of 3’Pool-seq reads is skewed towards the 3′-end of the gene body as expected. Normalized positions 0 and 100 correspond to 5′-end and 3′-end of genes, respectively