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Fig. 1 | BMC Genomics

Fig. 1

From: Fluorescent amplification for next generation sequencing (FA-NGS) library preparation

Fig. 1

FA-NGS Workflow: Following library preparation method of choice, amplification is applied with SYBR green mastermix, including polymerase (pink clouds), intercalating dye (green rectangles), and index primers (yellow, blue, purple rectangles). The fluorescence is monitored during library amplification. Melting curve analysis is then applied to an aliquot of the library to determine amplification success. If libraries pass the melting curve analysis QC, end RFU measurements are used to pool the amplified libraries in equal quantities. Libraries that fail melting curve analysis QC are omitted from subsequent steps. The pooled library is then purified and ready for NGS

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