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Fig. 3 | BMC Genomics

Fig. 3

From: Fluorescent amplification for next generation sequencing (FA-NGS) library preparation

Fig. 3

End RFU and transfer volumes of Nextera and AL library preparations: Equation for calculating transfer volumes from End RFU values, where Transfer Volumemax is determined by the user, and End RFUmin is the minimum End RFU value in the data set (a). Heatmaps of end RFU values and the corresponding calculated transfer volume of each well, 4 unique plasmids per quadrant of Nextera library prepared plasmids (b), 4 two-fold dilutions starting at 500 pg per quadrant of AL library prepared gDNA (c)

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