Fig. 4

Expression of the 20E and JH signals-related genes. a Transcriptomic analysis of the expression of the 20E and JH signals-related genes; b qRT-PCR analysis of the expression of the 20E and JH signals-related genes in the testes at indicated six developmental stages in X-axis. L6D0, L6D2, L6D4 and L6D6 represent day 0, 2, 4 and 6 of the 6th instar larval stage; P1D and P3D represent day 1 and 3 after pupation. 20E signals genes includes Ultraspiracle (USP), Ecdysone receptor (EcR), the nuclear hormone receptor 38 (HR38), the transcription factors betaFTZ-F1(βFTZ-F1), Broad Complex isform Z2/Z4 (BRC-Z2/Z4). JH signals genes includes Methoprene-tolerant (Met1) and Krüppel homolog (Kr-h1). The relative expression levels are normalized to the expression level of Slgapdh. One-way ANOVA analysis of variances was performed, followed by Turkey’s Multiple Comparison Test for significance analysis of the differences by GraphPad Prism 5 software. The values are mean ± SEM (n = 3). The significance is represented with different letters