Fig. 2

p53-bound regulatory elements influence cell proliferation. a p53 binding sites as determined by ChIP-Seq (black) and p53 consensus motifs (grey). b Distribution of distances to nearest annotated transcription start site for all sgRNAs in CRISPR library. c Distribution of number of sgRNA designs per p53 ChIP-Seq peak. d Distribution of number of sgRNA designs per p53 consensus motif. e Western blot analysis of dCas9-KRAB expression in 769P cells. f Comparison of log₂ fold changes (relative to pDNA) for all sgRNAs in CRISPR library between replicates. g Volcano plot comparing significance of sgRNA enrichment/depletion and log₂ fold change (relative to pDNA) for all sgRNAs in CRISPR library. h Visualization of enrichment/depletion for sgRNAs targeting a selected subset of peaks (red) compared to all sgRNAs in CRISPR library (black). i Comparison of log₂ fold change (relative to pDNA) and distance from nearest annotated TSS for all sgRNAs in CRISPR library