Fig. 3

Functional characterization of p53-bound regulatory elements that influence cell proliferation. a Schematic of p53 motifs and sgRNA targets located in Peak 2319. (ChromHMM track legend: red = active promoter; orange = strong enhancer) (b) Log₂ fold changes (relative to pDNA) in CRISPR screen for sgRNAs targeting Peak 2319. FDR values were calculated using the Benjamini-Hochberg method. c Schematic of p53 motifs and sgRNA targets located in Peak 384. (ChromHMM track legend: yellow = weak/poised enhancer) (d) Log₂ fold changes (relative to pDNA) in CRISPR screen for sgRNAs targeting Peak 384. FDR values were calculated using the Benjamini-Hochberg method. e Comparison of cellular growth rates following inhibition of Peak 2319 or Peak 384. P-values were calculated using the two-tailed unpaired Student’s t-test with equal variances. **P < 0.01, *P < 0.05