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Fig. 5 | BMC Genomics

Fig. 5

From: Identification of functional regulatory elements in the human genome using pooled CRISPR screens

Fig. 5

Functional characterization of p53-bound regulatory elements that influence cellular response to DNA damage. a Schematic of p53 motifs and sgRNA targets located in Peaks 974, 975, and 976. (ChromHMM track legend: red = active promoter; orange = strong enhancer; yellow = weak/poised enhancer; dark green = transcriptional transition/elongation; light green = weak transcribed) (b-d) Log2 fold changes (relative to pDNA) in CRISPR screen for sgRNAs targeting b Peak 975, c Peak 976, and d Peak 974. FDR values were calculated using the Benjamini-Hochberg method. e Schematic of p53 motifs and sgRNA targets located in Peak 685. f Log2 fold changes (relative to pDNA) in CRISPR screen for sgRNAs targeting Peak 685. FDR values were calculated using the Benjamini-Hochberg method. g Cell cycle analysis of DNA damage response following inhibition of Peak 975 or Peak 685. P-values were calculated using the two-tailed unpaired Student’s t-test with equal variances. **P < 0.01

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