Fig. 4

Mid-pointed maximum-likelihood (ML) phylogenetic trees of SH glycoprotein gene (a) G glycoprotein gene (b) and the full-length genome sequences (c) of viruses from Kenya and Zambia (marked in red), plus 138 other sequences (> 13 kb) retrieved from GenBank (Additional Table 3). Bootstrap support values (evaluated by 1000 replicates) are indicated along the branches. Genetic subgroups A1, A2a, A2b, B1, and B2, are indicated. Multiple sequence alignment was done using MAFFT and the ML phylogeny inferred using GTR + Γ nucleotide substitution model and ultrafast bootstrap approximation in IQ-TREE. The genotype B2 Sabana strain sequence (GenBank accession number HM197719) reported from a wild mountain gorilla in Rwanda is marked in blue. The scaled bar indicates nucleotide substitutions per site