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Fig. 4 | BMC Genomics

Fig. 4

From: Choice of library size normalization and statistical methods for differential gene expression analysis in balanced two-group comparisons for RNA-seq studies

Fig. 4

False positive rates estimated from simulated data with variable read depths given fixed sample sizes. Illustrated are the fractions of FPs estimated from the RLE, TMM, UQ and UQ-pgQ2 normalization methods using the exact test, QL F-test or Wald test for sample sizes of 5, 10, 15, 20, 25, 30, 35 and 40. Plots are based on 122 simulated data with the mean read depth of 30 million and SD of 3 (a-c) or 5 million (d-f); a mean read depth of 40 million and SD of 3 (g-i) or 5 million (j-l); a mean read depth of 50 million reads and SD of 3 (m-o) or 5 million (p-r)

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BMC Genomics

ISSN: 1471-2164

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