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Fig. 4 | BMC Genomics

Fig. 4

From: Gene expression identifies metabolic and functional differences between intramuscular and subcutaneous adipocytes in cattle

Fig. 4

Modified Minus Average (MA) plot of IMF versus SC to allow the visualisation of the real IMF signal, independent of the LD contamination. Here, we have colour coded each mRNA using a formal numerical approach such that the expression of those highlighted in red and yellow tones (above 0) and purple (below 0) are most likely derived from the IMF adipocytes themselves and not from contaminating LD muscle. To achieve the colour coding we exploited the difference in expression detected between the ‘pure’ IMF and ‘pure’ LD muscle samples. For example, if an mRNA has higher expression in IMF than in LD then we conclude it likely derives from the marbling adipocytes. The mRNA encoding CH25H exemplifies this logic. In addition to being higher in IMF than SC, it is also higher in IMF than LD. In terms of exact position on the plot, the mRNA encoding CH25H has an A value of 9.77 and an M value of 1.52

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