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Table 6 The numerical and biological outcomes of the single criteria approach we applied to first diagnose the impact of contaminating LD muscle on the IMF samples. The background list was composed of 34,228 genes including duplicates (14,477 unique). A number of these approaches based on high expression in IMF identify the presence of contaminating LD muscle

From: Gene expression identifies metabolic and functional differences between intramuscular and subcutaneous adipocytes in cattle

Single Query

Number of genes satisfying criteria (in brackets duplicates removed)

Functional enrichment for ‘muscle systems process’ (FDR q value)

LD > 4 fold higher than SC

279 (171)

2.04 e-39

IMF > 1.32 fold higher than the average of all fat depots

1833 (1112)

3.05 e-43

IMF > 1.68 fold higher than the average of all fat depots

741 (452)

4.42 e-44

IMF > 2 fold higher than SC

600 (380)

1.26 e-40

IMF > 1.32 fold lower than SC

890 (581)

None identified. Top hit ‘defense response’ 5.09 e-18

IMF > 2 fold higher than LD

329 (219)

None identified. Top hit ‘lipid metabolic process’ 2.98 e-4

IMF > LD by any amount

22,156 (10,679)

None identified. Top hit ‘cell migration’ 1.51 e-11

IMF < LD by any amount

12,072 (6839)

None identified. Top hit ‘translational termination’ 3.02 e-18