Fig. 2

Characterization of the mouse Zmat2 locus and gene. a. Schematic of the mouse Hars-Hars2- Zmat2 locus on chromosome 18. Exons are shown as lines and boxes (red for Hars, blue for Hars2, black for Zmat2); coding regions are solid and non-coding segments white. The direction of transcription is indicated for each gene and a scale bar is shown. b. Map of the mouse Zmat2 gene as depicted in the Ensembl genome database. Coding regions are in black and noncoding segments in white. A scale bar is shown. c. Mapping the beginning and end of mouse Zmat2: diagram of mouse Zmat2 exon 1 (left) and exon 6 (right), and graphs of gene expression data from the SRA NCBI RNA-sequencing library, SRX116916 (Additional file 1: Table S1), using 60 base pair genomic segments a-e, and a-f, respectively, as probes. The DNA sequence below the left graph depicts the putative 5′ end of exon 1, with the locations of the 5′ end of the longest RNA-sequencing clone indicated by a vertical arrow. Shown below the right graph is the DNA sequence of the putative 3′ end of exon 6. A potential polyadenylation signal (AATAAA) is underlined and a vertical arrow denotes the possible 3′ end of Zmat2 transcripts. d. Diagram of the mouse Zmat2 mRNA. Coding regions are in black and non-coding segments in white. The length is indicated in nucleotides (nt), as are the number of codons in the open reading frame. e. Schematic of the mouse ZMAT2 protein, with NH₂ (N) and COOH (C) terminal (term), and zinc finger (ZnF) regions labeled and color-coded