Fig. 6

Validation of RNA-seq data by qPCR. To validate the RNA-seq data, the relative mRNA levels of 17 randomly selected DEGs in the gills of hybrid tilapia were examined by qPCR. The mRNA levels by qPCR are presented as the fold change compared with the mock-treated control after normalization against β -actin. The relative expression levels from the RNA-seq analysis were calculated as log₂FC values. The gene nkai1 was calculated in two different groups. (Note: nkain1–1 was in a group C vs. A, nkain1–2 was in group C vs. SA; trpv4–1 was in group S vs. SA, trpv4–2 was in group C vs. S)