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Table 3 Primer and probe sequences

From: Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

Gene

Forward primer

Reverse primer

Probe

Application

gsdf

GGCAGCATTTCAGACCACTA

GACAAAGCAGTGGCTGTACC

TGCTGCAGGACCCTCAGCCTGG

qPCR

atttaggtgacactatagGGTGAGGGTGCTGAACTCAT

taatacgactcactatagggTGCCATGGAGAGTTGTTGAA

 

ISH probe synthesis

inha

TGGTGGCTCTCTCCTCTGAT

ATGAGCAAGTCATCCTCTTCC

CCAGCTCTGGCTCTACCTGTGATAGCT

qPCR

atttaggtgacactatagGTAGGTGGTCCAGCCATCAG

taatacgactcactatagggTTGGACTGGTTCAAACAGCA

 

ISH probe synthesis

  1. Primer and probe sequences (5′-3′) designed for gsdf and inha and used in this study. Sp6 and T7 sequences are shown in lower case letters.
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BMC Genomics

ISSN: 1471-2164

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