Fig. 2

Transfection and knockdown efficiency of shLuman on ESCs and the effects of Luman knockdown on decidualization of ESCs. a Transfection efficiency was observed by fluorescence microscopy in the shNC and shLuman transfected ESCs. b & c The Luman mRNA and protein expression level were detected in the shNC and shLuman groups. d The setup with Luman being silenced during different time point after decidualization induction. e-g mRNA expression of Luman, prl8a2, and prl3c1 in ESCs at 0, 24, 48, and 72 h of decidualization. P < 0.05 was considered significant. All data are represented as the mean ± SEM of repeated experiments (n = 3)