Fig. 4

Functional categorization of the 52 proteins identified upon MS analysis of the discriminative protein spots from 2-DE. a Overview of significantly enriched biological process-associated Gene Ontology categories, based on T. melanosporum annotation of the MS dataset proteins. Frequency data refers to cluster frequency ratio (black bars) and total frequency ratio (grey bars). Specifically, black bars represent the number of annotated proteins from the MS dataset associated with each GO term divided by the total number of identified and annotated proteins of the MS dataset, while gray bars represent the number of proteins in the T. melanosporum proteome reference set associated with each GO term divided by the total number of annotated proteins in the proteome reference set. The corrected FDR after statistical analysis is reported for each GO term. b Graphical description of the sulfur compound GO terms contained in the categories listed in panel A. Nodes, represented by circles, are shaded according to a p-value color coding obtained by statistical analysis. The range of the color scale varies from yellow (down-represented) to orange (over-represented)