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Fig. 6 | BMC Genomics

Fig. 6

From: Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environments

Fig. 6

Transcriptional regulation of sulfur VOC pathway genes in white truffle fruiting bodies. a Hierarchical clustering (Euclidean method) of genes related to sulfur metabolism. The heat map displays the Z-score of the identified transcripts, as measured in the RNA-seq analysis. On the top, significantly regulated genes across the pairwise comparisons (FDR < 1%) are shown in color, while non significant values (FDR > 1%) are shown as white cells. Induction or repression refer to the AL sample (internal standard in all pairwise comparisons). Red marked genes were further analyzed through qPCR. Additional information on the selected transcripts is reported in Supporting Information (see Additional file 12: Table S8). b Schematics of the sulfur VOC metabolic pathway derived from Martin et al. [22]. Numbers, indicating the enzyme catalyzing the specific reactions associated to each step, correspond to those listed in Supporting Information (see Additional file 13: Table S9). Coloured arrows mark those steps whose coding genes were analyzed by qPCR (see Additional file 14: Figure S4); conversely, grey arrows indicate not analyzed genes and the outcome of the measurements is visulized through different arrow colors, where orange represents genes up-regulated in AL samples and green those up-regulated in IS samples. c Relative profile plot of expression of the selected 19 genes across SM (red line), IS (green line) and AL samples (blue line). Median expression values are plotted and respective trend lines are shown. d Dendrogram representation of aggregative hierarchical clustering performed on the qPCR dataset

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