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Fig. 12 | BMC Genomics

Fig. 12

From: Transcriptome analyses of liver in newly-hatched chicks during the metabolic perturbation of fasting and re-feeding reveals THRSPA as the key lipogenic transcription factor

Fig. 12

Verification of differential expression of eight lipolytic genes using qRT-PCR analysis. Nutritional state is indicated by bar color, where green = fed, red = fasted and blue = refed condition. Values represent least-square means (LSM) and their standard error (LSE) of normalized expression of five cockerels (biological replicates) analyzed in duplicate. Expression levels, determined by qRT-PCR analysis, were normalized using the geNorm procedure in qBase software [59]. Values possessing different superscripts are significantly different as determined by analysis of variance (ANOVA) using the general linear models (GLM) procedure in Statistical Analysis System (SAS) software and with mean separation using Tukey’s Studentized Range Test

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