Fig. 13

Experimental design of the fasting and re-feeding perturbation in newly-hatched broiler chicks. Five newly-hatched male chicks were randomly assigned to 10 treatment groups (T1-T10). The chicks assigned to control fully-fed groups (D1FED24h or T2, D2FED48h or T4, D3FED72h or T7, and D4FED96h or T9) were provided with a commercial starter ration and water ad libitum from hatching (D0) until the time of tissue sampling. Fasting groups (D0FAST4h or T1, D1FAST24h or T3, and D2FAST48h or T5) of chicks were brooded with no access to feed (start after hatchling) for 4, 24 and 48 h, respectively. Chicks in the REFED groups (D2REFED4h or T6, D3REFED24h or T8 and D4REFED48h or T10) were fasted for 48 h and subsequently re-fed for 4, 24 and 48 h, respectively, prior to the time of tissue sampling