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Fig. 2 | BMC Genomics

Fig. 2

From: A systemic approach to screening high-throughput RT-qPCR data for a suitable set of reference circulating miRNAs

Fig. 2

Method of analyzing the stability of miRNA combinations. We decided to analyze combinations of miRNA from a dataset in a context of a dataset. For all possible combinations of miRNAs from a dataset, we sequentially appended an average of expressions of component miRNAs to a dataset (each sample had an additional entry with an average of expression of component miRNAs). Next step was to run the analysis in the same manner as for single miRNAs (as in Fig. 1b), which allowed to identify the average ranking value of a combination in a dataset. Then we removed the combination from the dataset and added another one to ensure that only one combination was present in the dataset at all time. This approach allowed us to aggregate the results from single and combinations of miRNAs without disrupting the workings of the stability measurement tools

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